Circulating levels of Bcl-2 and its expression in the nasal mucosa of patients with chronic rhinosinusitis

Iuliia Kalashnyk-Vakulenko 1, Galina Gubina-Vakulyck 2, Anatolii Onishchenko 3, Oksana Nakonechna 4, Tatyana Gorbach 4, Viktoriya Tkachenko 5, Anton Tkachenko 3 *
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1 Department of Otorhinolaryngology, Kharkiv National Medical University, Kharkiv, Ukraine
2 Department of Pathological Anatomy, Kharkiv National Medical University, Kharkiv, Ukraine
3 Research Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Kharkiv, Ukraine
4 Department of Biochemistry, Kharkiv National Medical University, Kharkiv, Ukraine
5 Department of Microbiology, Virology and Immunology, Kharkiv National Medical University, Kharkiv, Ukraine
* Corresponding Author
J CLIN MED KAZ, Volume 19, Issue 6, pp. 63-67. https://doi.org/10.23950/jcmk/12689
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ABSTRACT

Aim: To evaluate expression of anti-apoptotic Bcl-2 protein in the nasal tissue and its levels in blood serum of patients with chronic rhinosinusitis with (CRSwNP) and without nasal polyps (CRSsNP).
Material and methods: Expression of Bcl-2 in the sinonasal tissue and its levels in blood serum of patients with CRSsNP and CRSwNP were evaluated immunohistochemically and using ELISA, respectively.
Results: In patients with CRSsNP, Bcl-2 was overexpressed in nasal epithelial cells mainly in the atrophic regions. However, its upregulation was also observed in regions with epithelial cell proliferation. Immunostaining for Bcl-2 was stronger both in the stroma and epithelial lining compared with control subjects. The level of Bcl-2 in blood serum was elevated in both forms of chronic rhinosinusitis with a more pronounced increase in CRSwNP.
Conclusion: CRSsNP and especially CRSwNP are associated with overexpression of anti-apoptotic Bcl-2 in nasal epithelial cells and in the lamina propria against the background of elevated circulating concentrations of Bcl-2.
Keywords: chronic rhinosinusitis, nasal polyps, nasal epithelial cells, apoptosis, B-cell lymphoma 2, Bcl-2.

CITATION

Kalashnyk-Vakulenko I, Gubina-Vakulyck G, Onishchenko A, Nakonechna O, Gorbach T, Tkachenko V, et al. Circulating levels of Bcl-2 and its expression in the nasal mucosa of patients with chronic rhinosinusitis. J CLIN MED KAZ. 2022;19(6):63-7. https://doi.org/10.23950/jcmk/12689

REFERENCES

  • Sedaghat AR. Chronic rhinosinusitis. Am Fam Physician. 2017;96(8):500-506.
  • DeConde AS, Soler ZM. Chronic rhinosinusitis: Epidemiology and burden of disease. Am J Rhinol Allergy. 2016;30(2):134-9. https://doi.org/10.2500/ajra.2016.30.4297
  • Dennis SK, Lam K, Luong A. A review of classification schemes for chronic rhinosinusitis with nasal polyposis endotypes. Laryngoscope Investig Otolaryngol. 2016;1(5):130–134. https://doi.org/10.1002/lio2.32
  • Bachert C, Zhang L, Gevaert P. Current and future treatment options for adult chronic rhinosinusitis: Focus on nasal polyposis. J Allergy Clin Immunol. 2015;136(6):1431-1440. https://doi.org/10.1016/j.jaci.2015.10.010
  • Kim JY, Kim DK, Yu MS, Cha MJ, Yu SL, Kang J. Role of epigenetics in the pathogenesis of chronic rhinosinusitis with nasal polyps. Mol Med Rep. 2018;17(1):1219–1227. https://doi.org/10.3892/mmr.2017.8001
  • Tan BK, Min JY, Hulse KE. Acquired immunity in chronic rhinosinusitis. Curr Allergy Asthma Rep. 2017;17(7):49. https://doi.org/10.1007/s11882-017-0715-0
  • Tan BK, Chandra RK, Pollak J, Kato A, Conley DB, Peters AT, et al. Incidence and associated premorbid diagnoses of patients with chronic rhinosinusitis. J Allergy Clin Immunol. 2013;131(5):1350-60. https://doi.org/10.1016/j.jaci.2013.02.002
  • Cho SW, Kim DW, Kim JW, Lee CH, Rhee CS. Classification of chronic rhinosinusitis according to a nasal polyp and tissue eosinophilia: limitation of current classification system for Asian population. Asia Pac Allergy. 2017;7(3):121–130. https://doi.org/10.5415/apallergy.2017.7.3.121
  • Stevens WW, Ocampo CJ, Berdnikovs S, Sakashita M, Mahdavinia M, Suh L, et al. Cytokines in chronic rhinosinusitis. Role in eosinophilia and aspirin-exacerbated respiratory disease. Am J Respir Crit Care Med. 2015;192(6):682-94. https://doi.org/10.1164/rccm.201412-2278oc
  • Yang Y, Jiang G, Zhang P, Fan J. Programmed cell death and its role in inflammation. Mil Med Res. 2015;2:12. https://doi.org/10.1186/s40779-015-0039-0
  • Opferman JT, Kothari A. Anti-apoptotic BCL-2 family members in development. Cell Death Differ. 2018;25(1):37–45. https://doi.org/10.1038/cdd.2017.170
  • Kale J, Osterlund EJ, Andrews DW. BCL-2 family proteins: changing partners in the dance towards death. Cell Death Differ. 2018;25(1):65–80. https://doi.org/10.1038/cdd.2017.186
  • Hata AN, Engelman JA, Faber AC. The BCL2 Family: Key Mediators of the Apoptotic Response to Targeted Anticancer Therapeutics. Cancer Discov. 2015;5(5):475–487. https://doi.org/10.1158/2159-8290.cd-15-0011
  • Kalkavan H, Green DR. MOMP, cell suicide as a BCL-2 family business. Cell Death Differ. 2018;25(1):46–55. https://doi.org/10.1038/cdd.2017.179
  • Arya V, Singh S, Daniel MJ. Clinicopathological correlation of Bcl-2 oncoprotein expression in oral precancer and cancer. J Oral Biol Craniofac Res. 2016;6(1):18–23. https://doi.org/10.1016/j.jobcr.2015.12.011
  • Shukla S, Dass J, Pujani M. p53 and bcl2 expression in malignant and premalignant lesions of uterine cervix and their correlation with human papilloma virus 16 and 18. South Asian J Cancer. 2014;3(1):48–53. https://doi.org/10.4103/2278-330x.126524
  • Morawska-Kochman M, Śmieszek A, Marcinkowska K, Marycz KM, Nelke K, Zub K, et al. Expression of Apoptosis-Related Biomarkers in Inflamed Nasal Sinus Epithelium of Patients with Chronic Rhinosinusitis with Nasal Polyps (CRSwNP)-Evaluation at mRNA and miRNA Levels. Biomedicines. 2022;10(6):1400. https://doi.org/10.3390/biomedicines10061400
  • Fokkens WJ, Lund VJ, Mullol J, Bachert C, Alobid I, Baroody F, et al. EPOS 2012: European position paper on rhinosinusitis and nasal polyps 2012. A summary for otorhinolaryngologists. Rhinology. 2012;50(1):1–12. https://doi.org/10.4193/Rhino12.000
  • Ozturk AB, Bayraktar R, Gogebakan B, Mumbuc S, Bayram H. Comparison of inflammatory cytokine release from nasal epithelial cells of non-atopic non-rhinitic, allergic rhinitic and polyp subjects and effects of diesel exhaust particles in vitro. Allergol Immunopathol (Madr). 2017;45(5):473-481. https://doi.org/10.1016/j.aller.2016.10.015
  • Basinski TM, Holzmann D, Eiwegger T, Zimmermann M, Klunker S, Meyer N, et al. Dual nature of T cell-epithelium interaction in chronic rhinosinusitis. J Allergy Clin Immunol. 2009;124(1):74-80.e1-8. https://doi.org/10.1016/j.jaci.2009.04.019
  • Chand HS, Harris JF, Tesfaigzi Y. IL-13 in LPS-induced inflammation causes Bcl-2 expression to sustain hyperplastic mucous cells. Sci Rep. 2018;8(1):436. https://doi.org/10.1038/s41598-017-18884-9
  • Chand HS, Harris JF, Mebratu Y, Chen Y, Wright PS, Randell SH, et al. Intracellular insulin-like growth factor-1 induces Bcl-2 expression in airway epithelial cells. J Immunol. 2012;188(9):4581-9. https://doi.org/10.4049/jimmunol.1102673
  • Harris JF, Fischer MJ, Hotchkiss JA, Monia BP, Randell SH, Harkema JR, et al. Bcl-2 sustains increased mucous and epithelial cell numbers in metaplastic airway epithelium. Am J Respir Crit Care Med. 2005;171:764–772. https://doi.org/10.1164/rccm.200408-1108OC
  • Suo L, Zhao C, An Y, Zhang X, Tao Z. Expression of Bcl-2 mRNA and Bcl-2 protein in nasal mucusa of rat in allergic rhinitis model. Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2004;18(2):105-7 [in Chinese]
  • Mumbuc S, Karakok M, Baglam T, Karatas E, Durucu C, Kibar Y. Immunohistochemical analysis of PCNA, Ki67 and p53 in nasal polyposis and sinonasal inverted papillomas. J Int Med Res. 2007;35(2):237-41. https://doi.org/10.1177/147323000703500208
  • Campbell KJ, Tait SWG. Targeting BCL-2 regulated apoptosis in cancer. Open Biol. 2018;8(5):180002. https://doi.org/10.1098/rsob.180002