Assessment of Antibacterial and Antifungal Activity of a Biocomposite Based on Nanocellulose in Vitro
Madina Rashova 1 * ,
Zhanerke Amirkhanova 2,
Saule Akhmetova 3,
Berik Tuleubaev 4,
Dinara Turebekova 5,
Amina Koshanova 6,
Vladimir Vinokurov 7 More Detail
1 PhD student of the Karaganda Medical University, Karaganda, Gogol Street 40, Kazakhstan.
2 Associate Professor, Department of Biomedicine, "Karaganda Medical University", Karaganda, Gogol Street 40
3 Professor, Department of Biomedicine, "Karaganda Medical University", Karaganda, Gogol Street 40
4 Head of the Department of Surgical Diseases, "Karaganda Medical University", Karaganda, Gogol Street 40
5 . Junior Researcher, Research Laboratory, Institute of Life Sciences, "Karaganda Medical University", Karaganda, Gogol Street 40
6 Associate Professor, Department of Surgical Diseases, "Karaganda Medical University", Karaganda, Gogol Street 40,
7 Head of Department of Physical and Colloid Chemistry, Doctor of Chemical Sciences, Professor of the Federal State Autonomous Educational Institution of Higher Education “Gubkin Russian State University of Oil and Gas (National Research University)”, Moscow
* Corresponding Author
J CLIN MED KAZ, Volume 22, Issue 3, pp. 53-57.
https://doi.org/10.23950/jcmk/16328
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Author Contributions: Conceptualization, S.A., B.Т. and V.V.; methodology, S.A. and Zh.Т.; formal analysis, S.A., B.Т. and V.V.; resources, V.V.; writing – original draft preparation, M.R.; writing – review and editing, S.A., Zh.Т. and M.R.; visualization, M.R.; supervision, S.A., B.Т. and V.V.; project administration, funding acquisition, А.К.; statistical analysis, D.T. All authors have read and agreed to the published version of the manuscript.
Data availability statement: The corresponding author can provide the data supporting the study's conclusions upon request. Due to ethical and privacy constraints, the data are not publicly accessible.
ABSTRACT
Introduction: Biomaterials which used in the treatment of osteomyelitis, should not only fill in bone defects, but also be a “tool” for local delivery of antibiotics. The bone substitute is the biomaterial of human, animal, plant or synthetic genesis, implanted into the body to restore and strengthen bone substance. The creation of the latest nanocompositional materials today is the main direction of the development of science and technology. Nanocellulose (NC) and biocomposits based on it became one of the most promising “green” materials due to its unique properties. Our hypothesis: nanocellulose-based biocomposites with added antibiotics show antimicrobial effectiveness in vitro.
Methods: For preclinical testing, three samples of the NC were provided: nanofibrillar, nanocrystalline, microfibrillar nanocellulose. The microbiological purity was studied, the minimum-inhibitory concentration of the antibiotic, and the study of the antibacterial and antifungicide effect of biocomposite samples by the method of museum tests of Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 25922), Bacillus subtilis (ATCC 6633), Candida Albicans ( ATCC 10231) by measuring the diameter of the growth retardation zone.
Results: Biocomposites based on nanocellulose, saturated with antibiotics, demonstrate high antibacterial and antifungicidal potential. Further experimental studies are required by their ability to uniform and slow controlled release of the active substance, along with the effective suppression of the growth of microorganisms and a prolonged effect, which would allow them to be considered as an effective local transport system. In the future, it is planned to implant biocomposites with an antibiotic in the treatment of osteomyelitis in vivo.
CITATION
Rashova M, Amirkhanova Z, Akhmetova S, Tuleubaev B, Turebekova D, Koshanova A, et al. Assessment of Antibacterial and Antifungal Activity of a Biocomposite Based on Nanocellulose in Vitro. J CLIN MED KAZ. 2025;22(3):53-7.
https://doi.org/10.23950/jcmk/16328
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