Investigation of LncRNAs expression in patients with hepatitis B virus

Cansu Önlen Güneri 1 * , Hamza Malik Okuyan 2, Gülay Gülbol Duran 3, Mehmet Demir 4
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1 1Department of Medical Laboratory Techniques, Gulhane Vocational School of Health Services, University of Health Sciences, Ankara, Turkey
2 Faculty of Health Sciences, Sakarya University of Applied Sciences, Sakarya, Turkey
3 Basic Medical Sciences, Department of Medical Biology, Faculty of Medicine, Mustafa Kemal University, Hatay, Turkey
4 Internal Medicine Sciences, Department of Gastroenterology, Faculty of Medicine, Mustafa Kemal University, Hatay, Turkey
* Corresponding Author
J CLIN MED KAZ, In press.


Aim: Patients infected with the hepatitis B virus (HBV) are at a higher risk of cirrhosis and hepatocellular carcinoma. Despite antiviral therapy’s evolution, many patients still cannot respond to existing therapies. Hence, to detect the changes in liver function earlier, non-invasive methods are needed. Long noncoding RNAs (LncRNAs) plays important roles in essential biological process as well as human cancer. LncRNAs may be used as biomarkers in human diseases. Thus, in this study, we purposed to analyze the expression levels of LncRNAs (HOX transcript antisense RNA (HOTAIR), maternally expressed 3 (MEG-3), highly up-regulated in liver cancer (HULC)) in patients with hepatitis B virus and healthy volunteers.
Material and methods: We chose three lncRNAs as candidate lncRNAs based on their association with liver disease. Whole Blood samples were collected from 40 patients with HBV and 48 healthy volunteers. The expression levels of all the samples were evaluated by quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR). Statistical analysis was implemented using GraphPad Prism software. P-value lower than 0.05 was statistically meaningful.
Results: The expression levels of HOTAIR and HULC were remarkably up-regulated in the plasma of the patients with HBV compared with healthy control, and LncRNAs exhibited more than two-fold increased (p<0.05). In contrast, MEG-3 expression levels were close to normal (control group).
Conclusion: Our findings showed that the expression of HOTAIR and HULC in plasma might be used as a new promising diagnostic and/or prognostic biomarker for HBV.


Önlen Güneri C, Okuyan HM, Gülbol Duran G, Demir M. Investigation of LncRNAs expression in patients with hepatitis B virus. J Clin Med Kaz. 2022.


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