Mutation analysis of TP53 in colorectal cancer, Peshawar, Khyber Pakhtunkhwa, Pakistan.

Ihsan Ali 1, Aamna Fayyaz 2, Zahid Anwar 3, Irfan Saif 4, Ikram Khan 4, Muhammad Jahangir 5, Mian Adnan Kakakhel 4 *
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1 College of Life Science, Northwest University, Xi’an, Shaanxi province, China
2 Department of Zoology, Kohat University of Science and Technology, Kohat, Khyber Pakhtunkhwa, Pakistan
3 Animal Sciences Institute, Faculty of Animal Genomics and Biotechnology, PARC Institute of Advanced Studies in Agriculture, National Agricultural Research Center, Islamabad, Pakistan
4 MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China
5 National Clinical Research Center for Mental Disorders, Xiangya School of Medicine, Central South University, Changsha, Hunan, China
* Corresponding Author
J CLIN MED KAZ, Volume 18, Issue 2, pp. 25-30. https://doi.org/10.23950/jcmk/9719
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ABSTRACT

Aim: Colorectal cancer is a kind of solid tumor and third most common of cancer which leads to death. It is a heterogeneous disease characterized by genetic and epigenetic aberrations. The present study was aimed to reveal the mutation at TP53 in Peshawar Pakistan.
Material and methods: For this purpose, a total of 50 blood samples in EDTA tubes was collected from Peshawar with complete information of patients using questionnaire. The polymerase chain reaction was performed.
Results: The results of the present study have revealed that the ratio of colorectal cancer in males is higher 62% than females (38%). Histopathological results have indicated that moderately differentiated adenocarcinoma is 25 (50%) followed by poorly differentiated adenocarcinoma 15 (30%), Well-differentiated adenocarcinoma 8 (16%), and Metastatic adenocarcinoma 2 (4%) in 50 colorectal cancer patients. The nucleotide sequence of the human TP53 gene of colorectal cancer patients was aligned with the human wild-type TP53 database sequence with NC_191170 (NCBI) using CLUSTALW. After BLASTING, the mutation was found in exon 5 and exon 7 of TP53, while no mutation was found in exon 8 of TP53. That patient in which point mutation was found in exon 5 and 7 respectively showed less survival as compared to that of colorectal cancer patients having no point mutation in the TP53 gene.
Conclusion: The present study concluded that point mutations were found in exons 5&7 of the TP53 gene and patients having TP53 gene mutations shown less survival rate compared to colorectal cancer patients having no mutations.

CITATION

Ali I, Fayyaz A, Anwar Z, Saif I, Khan I, Jahangir M, et al. Mutation analysis of TP53 in colorectal cancer, Peshawar, Khyber Pakhtunkhwa, Pakistan.. J CLIN MED KAZ. 2021;18(2):25-30. https://doi.org/10.23950/jcmk/9719

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