Study of serum microRNA-217 expression pattern in different stages of Diabetic Kidney Disease - a cross-sectional study
Ponnudhali D 1,
Lakshmiprabha S 2,
Priya K Dhas 3,
Nagarajan P 4 * More Detail
1 Professor, Department of Biochemistry, Vinayaka Missions’ Kirupananda Variyar Medical College & Hospital, Vinayaka Missions Research Foundation (DU), NH-47, Sankari Main Road, Salem, Tamil Nadu
2 Associate Professor , Department of Biochemistry, KMMC Medical College and Hospital, Muttom, Kanyakumari, 629202
3 Vinayaka Mission's Kirupananda Variyar Medical College, Vinayaka Missions Research Foundation (Deemed to be University), Salem, Tamilnadu.
4 Professor &HOD, Dept of Nephrology, Govt. Mohankumaramangalam Medical College & Hospital, Salem-1, Tamil Nadu.
* Corresponding Author
J CLIN MED KAZ, In press.
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ABSTRACT
Background: Diabetic kidney disease (DKD) represents the leading cause of end-stage renal disease worldwide, affecting 30-40% of patients with type 2 diabetes mellitus (T2DM). Some microRNAs (miRNAs), like miRNA-217 are upregulated in Diabetic Kidney Disease (DKD), and known to play a key role in Diabetes Mellitus & its complications. Recent studies have shown miRNA 217 to be involved in the development of DKD, by promoting renal inflammation, angiogenesis and fibrosis.
Aim & objectives: To assess the miR217 expression levels in DKD patients with differing stages of albuminuria, compared to normoalbuminuric Diabetes mellitus -II patients & to identify its role as a predictive biomarker of DKD.
Methodology: This analytical cross-sectional study recruited 135 participants aged 40-65 years from a Govt medical college Hospital in Tamil Nadu. Participants includedT2DM patients (n=34) & DKD patients & healthy controls(n=35). Fasting serum samples were collected & miRNA-217 extraction and qRT-PCR quantification was done & Fold change was calculated, along with the baseline clinical parameters (age, BMI, HbA1c, lipids, urea, creatinine, eGFR, UACR).
Statistical analyses like Kruskal-Wallis tests, Spearman's correlations & ROC curves were computed using Jamovi software version 2.6.44
Results: Serum miRNA-217 expression was markedly elevated in DKD patients with microalbuminuria (39.36 ± 76.24 fold change) & macroalbuminuria (64.32 ± 109.23 fold change) compared to Diabetes mellitus -II patients with normoalbuminuria (2.29 ± 4.77, P=0.027).MiRNA-217 correlated positively with UACR (ρ=0.472, p<0.001) and was able to discriminate DKD from Diabetes-II, with 59% sensitivity & 90% specificity, ROC analysis yielded AUC 0.738 (p<0.001).
Conclusion: Serum miRNA-217 exhibits progressive up-regulation in accordance with stages of DKD severity, with moderate diagnostic utility. miRNA-217 could be a potential biomarker for early DKD detection and risk stratification, warranting longitudinal studies in larger populations for further validation and establishment of these findings.
CITATION
D P, S L, Dhas PK, P N. Study of serum microRNA-217 expression pattern in different stages of Diabetic Kidney Disease - a cross-sectional study. J Clin Med Kaz. 2026.